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Novus Biologicals
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FOXPRO Inc
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ATCC
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Image Search Results
Journal: Frontiers in Physiology
Article Title: The Endothelial Mechanotransduction Protein Platelet Endothelial Cell Adhesion Molecule-1 Is Influenced by Aging and Exercise Training in Human Skeletal Muscle
doi: 10.3389/fphys.2018.01807
Figure Lengend Snippet: (A–D) Changes in relative phosphorylation of eNOS and mechanosensor proteins from rest to passive leg movement conducted before and after the training period. Relative changes in (A) endothelial nitric oxide synthase serine 1177 phosphorylation (eNOS p117 ), (B) vascular endothelial growth factor receptor 2 serine 1177 (VEGF-R2 ser1177 ), (C) vascular endothelial cadherin tyrosine 731 (VE CAD tyr731 ), and (D) platelet endothelial adhesion molecule 1 tyrosine 713 (PECAM-1 tyr713 ) from young and old subjects before and after the training intervention. $ denotes a significant change from rest to passive leg movement, ∗ denotes difference from before training within group. Data are mean ± SD. (E) Representative western blots of mechanosensor proteins. Western blots from one young and one old subject, before and after training of VEGF-R2 ser1177 , VE CAD tyr731 and PECAM-1 tyr713 . Samples are run in dublicats and presented together with loading control glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Note that changes in eNOS phosphorylation are obtained from immunohistochemical staining of individual microvessels within cross sections of muscle biopsies while the mechanosensor proteins reflect changes in whole muscle homogenates. Please see the Materials and Methods section and Figure for representative images.
Article Snippet: After gel electrophoresis, the proteins were transferred (semidry) to a polyvinylidene difluoride membrane (Immobilon Transfer Membrane, Millipore), which was incubated with ∼10 ml of primary antibody over-night and then washed three times for 5 min in Tris-buffered saline-Tween before incubation with secondary antibody for 1 h. The membranes were incubated with the following primary antibodies: VEGF-R2 (sc-19530; Santa Cruz Biotechnology, Santa Cruz, CA, United States); VEGF-R2 Tyr1175 (2478, Cell Signaling Technology, Danvers, MA, United States); eNOS (BD 610287, BD Biosciences, San Jose, CA, United States; eNOS ser1177 (Calbiochem 482737, Merck Milipore, Darmstadt, Germany); PECAM-1 (AF806, R&D Systems Inc., Minneapolis, MN, United States); PECAM-1 Tyr713 (BS4666, Bioworld Technology, St. Louis Park, MN, United States); VE-cadherin (ab33168, Abcam, Cambridge, United Kingdom);
Techniques: Phospho-proteomics, Western Blot, Control, Immunohistochemical staining, Staining
Journal: Heliyon
Article Title: The clinical impact of mRNA therapeutics in the treatment of cancers, infections, genetic disorders, and autoimmune diseases
doi: 10.1016/j.heliyon.2024.e26971
Figure Lengend Snippet: List of all conducted clinical trials for mRNA-based treatment of cancers (Until Jan 2024).
Article Snippet: 112 , NCT04455620 , Encodes a modified
Techniques: Clinical Proteomics, In Vivo, Ex Vivo, Immunopeptidomics, Adjuvant, Isolation, Membrane, Mutagenesis, Transplantation Assay, Derivative Assay, Comparison, Vaccines, Knock-Out, Modification