Journal: Frontiers in Physiology

Article Title: The Endothelial Mechanotransduction Protein Platelet Endothelial Cell Adhesion Molecule-1 Is Influenced by Aging and Exercise Training in Human Skeletal Muscle

doi: 10.3389/fphys.2018.01807

Figure Lengend Snippet: (A–D) Changes in relative phosphorylation of eNOS and mechanosensor proteins from rest to passive leg movement conducted before and after the training period. Relative changes in (A) endothelial nitric oxide synthase serine 1177 phosphorylation (eNOS p117 ), (B) vascular endothelial growth factor receptor 2 serine 1177 (VEGF-R2 ser1177 ), (C) vascular endothelial cadherin tyrosine 731 (VE CAD tyr731 ), and (D) platelet endothelial adhesion molecule 1 tyrosine 713 (PECAM-1 tyr713 ) from young and old subjects before and after the training intervention. $ denotes a significant change from rest to passive leg movement, ∗ denotes difference from before training within group. Data are mean ± SD. (E) Representative western blots of mechanosensor proteins. Western blots from one young and one old subject, before and after training of VEGF-R2 ser1177 , VE CAD tyr731 and PECAM-1 tyr713 . Samples are run in dublicats and presented together with loading control glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Note that changes in eNOS phosphorylation are obtained from immunohistochemical staining of individual microvessels within cross sections of muscle biopsies while the mechanosensor proteins reflect changes in whole muscle homogenates. Please see the Materials and Methods section and Figure for representative images.

Article Snippet: After gel electrophoresis, the proteins were transferred (semidry) to a polyvinylidene difluoride membrane (Immobilon Transfer Membrane, Millipore), which was incubated with ∼10 ml of primary antibody over-night and then washed three times for 5 min in Tris-buffered saline-Tween before incubation with secondary antibody for 1 h. The membranes were incubated with the following primary antibodies: VEGF-R2 (sc-19530; Santa Cruz Biotechnology, Santa Cruz, CA, United States); VEGF-R2 Tyr1175 (2478, Cell Signaling Technology, Danvers, MA, United States); eNOS (BD 610287, BD Biosciences, San Jose, CA, United States; eNOS ser1177 (Calbiochem 482737, Merck Milipore, Darmstadt, Germany); PECAM-1 (AF806, R&D Systems Inc., Minneapolis, MN, United States); PECAM-1 Tyr713 (BS4666, Bioworld Technology, St. Louis Park, MN, United States); VE-cadherin (ab33168, Abcam, Cambridge, United Kingdom); VE-cadherin Tyr731 (NBP1-51393, Novus Biologicals, Littleton, CO, United States).

Techniques: Phospho-proteomics, Western Blot, Control, Immunohistochemical staining, Staining

Journal: Heliyon

Article Title: The clinical impact of mRNA therapeutics in the treatment of cancers, infections, genetic disorders, and autoimmune diseases

doi: 10.1016/j.heliyon.2024.e26971

Figure Lengend Snippet: List of all conducted clinical trials for mRNA-based treatment of cancers (Until Jan 2024).

Article Snippet: 112 , NCT04455620 , Encodes a modified IL-2 (BNT151) , In vivo , Solid Tumors , Monotherapy Combination with Anti-cancer Agents , Phase I/II (2021) , 84 , Adults (≥18) , BioNTech SE , United States Spain United Kingdom , Recruiting.

Techniques: Clinical Proteomics, In Vivo, Ex Vivo, Immunopeptidomics, Adjuvant, Isolation, Membrane, Mutagenesis, Transplantation Assay, Derivative Assay, Comparison, Vaccines, Knock-Out, Modification

Journal: The British Journal of Radiology

Article Title: Fetal MRI at 3T—ready for routine use?

doi: 10.1259/bjr.20160362

Figure Lengend Snippet: Imaging parameters of sequences used in fetal MRI at 3.0 T (Achieva, Philips Medical Systems, Best, Netherlands). These are guidelines that must be adapted to the corresponding scanner

Article Snippet: Tissue heating is also increased at 3.0 T, 44 albeit this heating is not dangerous for the fetus, it is uncomfortable for the pregnant female. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Sequence FOV (mm) Matrix TR (ms) TE (ms) Flip angle (degree) Slice thickness/gap (mm) Number of slices Acquisition time (s) Main indications Survey T 1 /TFE 450 256 × 128 8 2.3 15 5–8/0 31 41 Fetal position Survey T 2 /TSE 400 268 × 200 Shortest/3.5 Shortest/1.75 60 6/0 31 82 Fetal position T 1 ultrafast GE FS 250 168 × 153 Shortest/3.12 1.49 10 1.5–3/0 31 21 Brain: haemorrhage, calcifications T 1 ultrafast GE FS 375 252 × 230 Shortest/3.32 1.57 10 4/0 27–40 15–21 Body: thyroid, kidneys, digestive system (meconium) T 1 ultrafast GE 250 168 × 153 Shortest/3.12 1.49 10 3/0 31 14 Haemorrhage, calcifications, fat T 2 single-shot ultrafast SE 250 228 × 204 Shortest/5500–9800 200 90 2–3/0 25–32 25–35 Brain: anatomy, CSF T 2 single shot ultrafast SE 280 256 × 229 Shortest/5400–8750 100 90 3–4/0 32–48 24–37 Body: lung development, stomach, airway T 2 SSFP 250 168 × 125 Shortest/3.7 Shortest/1.83 90 6/−3 21 43 Brain: cysts T 2 SSFP 400 268 × 200 Shortest/3.8 Shortest/1.90 90 4–6/0 21 38 Body: vessels, heart FLAIR 230 112 × 89 7500 63.1 – 3.5/1 15–25 45 Brain: lamination, content of cystic lesions GRE EPI 250 120 × 115 1000 35 90 2–3/0 23–31 8–16 Bone/cartilage, haemorrhage DWI ( b = 700) 400 132 × 134 4600 Shortest/65 90 5/0.5 84 78 Body: kidneys, haemorrhage DWI ( b = 700) 230 152 × 106 2247 Shortest/74 90 4/1.17 24 24 Brain: ischaemia, haemorrhage SSFP Dynamic 450 212 × 281 2.8 1.39 90 10–30/2.35 120 (6 fps) 28 Fetal movements, swallowing T 1 FFE dynamic 300 152 × 149 Shortest/3.1 Shortest/1.44 10 2 8 (6 fps) 37 Bowel movements Single-shot MRCP 450 428 × 341 9449 487 90 40 9 106 3D fetus and surroundings DTI (6 directions) 250 135 × 96 Shortest/1400 Shortest/112 90 4/0 15 106 Tractography DTI (15 directions) 250 135 × 96 Shortest/856 Shortest/112 90 4/0 10 57 Tractography Spectroscopy – 12 × 12 2000 144 or shorter – – – 164 Metabolite measurement BOLD 230 96 × 94 907 35 90 4 15 50 fMRI, placenta Open in a separate window 3D, three-dimensional; BOLD, blood oxygen level dependent; CSF, cerebrospinal fluid; DTI, diffusion tensor imaging; DWI, diffusion-weighted imaging; EPI, echoplanar imaging; FFE, fast field echo; FLAIR, fluid-attenuated inversion recovery; fMRI, functional MRI; FOV, field of view; FS, fat saturated; GE, gradient echo; GRE, gradient recalled echo; MRCP, magnetic resonance cholangiopancreatography; SE, spin echo; SSFP, steady-state free-precession; TE, echo time; TFE, turbo field echo; TR, relaxation time; TSE, turbo spin echo.

Techniques: Imaging, Spectroscopy